19th Annual New England Biolabs Molecular Biology Summer Workshop

Smith College
Session 1, June 6-19, 2004

Day 1
Day 2 AM: some definitions; how to construct a genomic library
Day 2 PM: introduction to lambda bacteriophage
Day 2 Evening: all about restriction enzymes
Day 3 AM: the lac operon of E. coli; blue-clear selection
Day 3 PM: how to screen a library for a gene of interest; introduction to PCR
Day 4 AM: reverse-transcriptase PCR
Day 4 PM: PCR details; melting temperature
Day 5 PM: primer design considerations; how to confirm identity of PCR products
Day 6 AM: subcloning to a plasmid; quantitative real-time PCR
Day 6 PM: cDNA library construction; unidirectional cloning
Day 7 AM: preventing PCR contamination
Day 9 AM: interpreting spectrophotometry data; DNA fingerprint analysis
Day 9 PM: making a complete genomic library; more about lambda phage
Day 9 Evening: how Sanger sequencing works
Day 10 AM: plasmid, BAC and YAC cloning vectors; prokaryotic gene expression
Day 10 PM: eukaryotic gene expression; automated DNA sequencing
Day 10 Evening: obtaining sequencing primers; sequencing tricks for dealing with compressions
Day 11 AM: screening cDNA libraries
Day 12 AM: primers and protein expression
Day 12 PM: screening a cDNA library; studying single gene expression
Day 12 Evening: follow-ons to the Human Genome Project
Day 13 AM: Southern blot hybridization; microarray analysis
Day 13 PM: RNA interference
Day 14 AM: understanding Southern blot digest
Random notes on laboratory practices
Lab and lecture topics

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